Klf2 shrna
WebWe further show using KLF2 shRNA that the inhibitory eect of NMP on endothelial inammation and subsequent monocyte adhesion is KLF dependent. Enhancing KLF expression and activity improves WebMethods: Genetic manipulations of KLF2 were performed in bEnd.3 cells and SCI model. Western blot, qRT-PCR, immunofluorescence staining and Lyso-Tracker Red staining, Evans blue dye extravasation, behavioral assessment via Basso mouse scale (BMS), electrophysiology and footprint analysis were performed.
Klf2 shrna
Did you know?
WebMay 7, 2024 · These results confirm that this dual transduction protocol does not affect A20's ability to increase KLF2, KLF4, and eNOS expression levels. Transduction of HCAEC … WebNov 22, 2024 · Endothelial–mesenchymal transition occurs during intimal hyperplasia and neointima formation via mechanisms that are incompletely understood. Endothelial MAPK7 signaling is a key mechanosensitive fac...
WebSep 8, 2015 · Klf2 and Tfcp2l1 Knock Down Impairs 2i-Mediated mESC Self-Renewal (A) qRT-PCR analysis of Klf2 expression in Klf2 shRNA knockdown 46C mESCs. Data represent mean ± SD of three biological replicates. ∗∗ p < 0.01 versus scramble shRNA. (B) Western blot analysis of KLF2 expression in Klf2 shRNA knockdown 46C mESCs. Webactivity of KLF2 and ATF2 transcription factors, resulting in quiescent and healthy endothelium.3–7 However, as other molecular factors may also play a role, we recently established the specific gene expression in endothelium overlaying human atherosclerotic lesions, to identify genes with a potential to modulate intimal accumulation of ...
WebShRNA of mouse Klf2 were inserted into pLKD-CMV-G and PR-U6-shRNA vector. The sequences of shRNA are listed in Table S3. Lentiviral package Human embryonic kidney (HEK) 293T cells (ATCC, CRL-1573) were transfected with the vector plasmid, the lentiviral packaging constructs pCMV.DR8 and pMD2.G (Plasmid #12259, Addgene). WebNov 23, 2024 · Background: Circular RNAs (circRNAs), as a class of endogenous RNAs, are implicated in osteosarcoma (OS) progression. However, the functional properties of circDOCK1 in OS have been largely unexplored. The present study demonstrated the regulatory mechanism of circDOCK1 in OS. Methods: QRT-PCR and Western blots were …
WebHuman KLF2 (10365), Mouse Klf2 (16598), Rat Klf2 (306330), chicken... Summary: Kruppel-like factors (KLFs) are a family of broadly expressed zinc... Related Products: Antibodies …
WebJul 28, 2024 · Overexpression of KLF2 by adenovirus KLF2 (Ad-KLF2) in HUVECs reversed the inhibitory effect of OSS on UCP2 mRNA expression , suggesting that KLF2 can increase UCP2 expression. However, … hdd temperaturenWebStart gene knockdown with ready-to-use Klf2 mouse shRNA lentivirus , then verify knockdown efficiency with unique designed RT-PCR primers. Lentiviral Klf2 mouse shRNA … hddtemp debian packageWebSilencing of KLF2 with KLF2 shRNA abolished the effects of laquinimod in inhibiting the expression of endothelial adhesion molecules and attachment of U937 monocytes to … hdd taken apartWebFeb 10, 2008 · Normal undifferentiated ES colonies with positive alkaline phosphatase staining were maintained after knockdown using two shRNA constructs targeting different regions of Klf2 or Klf5, respectively. hdd ssd kopyalama programıWebDiabetic mice injected with KLF2 shRNA-lentivirus or control virus were treated with 20 mg/kg phloretin. Glucose, lipid profile, aortic atheroma, and endothelial nitric oxide synthase (eNOS) expression were detected. Phloretin retained endothelial function by KLF2-eNOS activation under hyperglycemia. hdd temperature rangehdd serial ata/ 600WebApr 15, 2024 · Western blot was performed to analyze the effects of KLF2 shRNA on knockdown of KLF2 expression and p15 and p21 expression in cells. We found that KLF2 … hdd si ssd wikipedia