Fk2 antibody

WebClone FK2 is the most widely used ubiquitin antibody. This clone reacts with mono- and polyubiquitinated conjugates, but does not react with free ubiquitin. 1, 2 WARNING This product is not for human or veterinary use. Certificates of Analysis & Batch Specific Data Get Batch-Specific Data and Documents by Batch Number WebBlock: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody (SMC-550) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti …

Mouse Anti-Ubiquitin Monoclonal Antibody (FK2), Conjugated

WebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be … WebClone FK2 is the most widely used ubiquitin antibody. This clone reacts with mono- and polyubiquitinated conjugates, but does not react with free ubiquitin. 1 , 2 WARNING This … flinders university tuition fees https://msledd.com

Anti-Multi Ubiquitin mAb(Monoclonal, FK2) Antibody MBL …

WebImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT. WebSep 13, 2024 · The FK2 monoclonal ubiquitin antibody is a frequently used and widely-cited ubiquitin antibody that detects both mono- and polyubiquitin, but not free ubiquitin. … WebApr 14, 2024 · Ubiquitinated proteins, detected by the monoclonal antibody FK2 (ub-FK2), and RNF8-dependent ubiquitinated H2A (ub-H2A, detected by monoclonal antibody E6C5) are localized on the sex body area (Lu et al., 2010). K48- and K63-linked poly-ubiquitins are enriched in the XY body (Lu et al., 2010). The role of ubiquitination around the XY body ... flinders university tonsley

Cyclin-Dependent Kinase 5 Regulates PSD-95 Ubiquitination in …

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Fk2 antibody

Fk2 antibody Sigma-Aldrich

WebMar 20, 2024 · Ubiquitin staining was performed using anti-ubiquitin (FK2) antibody. The blue (Hoescht 33342) but not red bacteria are L. dumoffii. Ubiquitin was commonly associated with LpCVs (arrows), but was ... WebSep 15, 2013 · The FK2 antibody is a mouse monoclonal recognizing both monoubiquitinated and polyubiquitinated conjugates. According to the manufacturer’s data sheet the FK2 antibody can recognize various types of ubiquitin chains; however, it is unknown if it has equal affinities for all seven ubiquitin chain linkages and for different …

Fk2 antibody

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Web(BML-PW8810-0500) Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2), 500 µg - Enzo Life Sciences - CiteAb 35 citations – A mouse monoclonal … WebGeneral description. Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated proteins. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated ...

WebDownload Zoom This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References Data Sheet … WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS. 分析证书.

WebWestern blots were developed using PVDF membranes and A-106 anti-ubiquitin primary antibody at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) …

WebMouse Monoclonal Antibody Shop MilliporeSigma™ anti-Ubiquitin Clone: FK2, at Fishersci.com. Fisher Scientific ; ... All Primary Antibodies ST1200100UG Print …

WebAug 17, 2011 · The FK1 and FK2 monoclonal antibodies are used to differentiate between monoubiquitination and polyubiquitination, since FK2 detects both monoubiquitinated and polyubiquitinated proteins, while FK1 recognizes only polyubiquitinated proteins (Fujimuro et al., 1994). Therefore, to assess whether the ubiquitination of PSD-95 reflects ... flinders uni workdayWebSep 10, 2016 · Scheme of the ELISA assay. (a) In the “forward” approach, microwell plates are coated with a polyclonal anti-EGFR intra, which captures the receptor from the lysate (1).Detection of Ub-EGFR or total EGFR (to normalize) is performed with primary monoclonal antibodies directed against Ub (FK2, 1) or EGFR extra (2), respectively, … flinders university up the hill programWebJan 7, 2024 · The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterized by one-dimensional Western blotting. The immunoglobulin … greater expectations ministries dothan alWebUbiquitin modification of p62 was detected using an antibody recognizing mono- and poly-ubiquitin (FK2) (lower panels). The EGFP–TRIM proteins were detected using an anti-GFP antibody. * indicates mono-ubiquitylated p62 and a high-molecular-mass p62. greater expectations phlWebThe FK2 antibody works great in both human and rat samples (I haven't tried mouse) by western blot and immunofluorescence. This antibody should recognize the ubiquitin modifications (both mono... greater expectations njWebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin … flinders university train stationWebThe Tb-anti-ubiquitin-FK2 antibody preferentially binds to mono- or poly-ubiquitinated proteins over free ubiquitin. This antibody can be used in combination with purified GFP … greater elizabeth chamber of commerce nj