Cisplatin a549 ic50

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August undefined, 2024

WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … WebThe treatment of A549 cells with CLEFMA improved cisplatin efficacy and decreased cisplatin ototoxicity by regulating apoptosis-inducing factor and caspase-12 [12]. CLEFMA elicits apoptotic cell ...

The 50 % inhibitory concentration (IC50) of cisplatin in …

WebFeb 28, 2024 · Complexes 36–40 were tested against lung carcinomas A549 and A549/DDP, cisplatin-insensitive cells, and normal healthy (HL-7702) cells. ... (IC50 = 3.87 μM), complex 40 (IC 50 = 0.91 μM), and cisplatin (IC 50 = 50.12 μM), all of which showed lower cytotoxic effects against HL-7702 compared with the standard approved regimen … phlebotomy side effects mayo clinic

Glutathione Peroxidase 1 Promotes NSCLC Resistance to Cisplatin …

WebIC50 values of cisplatin were determined for A549/SEN and A549/CR cells using inhibition dose-response curves with variable slopes. For the colony formation assay, 500 cells were seeded in 12-well plates and cultured in … WebNov 19, 2013 · A549 cells were exposed to cisplatin at an IC50 dose (12 µM) for 24 h; then, cells were co-stained with Annexin V and MitoSox and analyzed by flow cytometry. (A-C) Annexin V-negative subpopulation (about 15-20%) was gated in each sample in order to exclude the apoptotic fraction of cells from the analysis. WebMar 22, 2024 · a A549 and A549/DDP cells were exposed to different concentrations of cisplatin (1, 10, 20, 40, 80, 160 μM) for 48 h, followed by the determination of cell … phlebotomy simplified

Gleevec (STI-571) inhibits lung cancer cell growth (A549) and ...

Hypoxia-induced autophagy mediates cisplatin resistance in

WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. WebIn A549 cells, the IC50 concentration of cisplatin resistant cells was determined as 23.60 µM compared to 1.58 µM in the original parent cell line, a 15-fold increase in the … t stock t store locationWebMar 22, 2024 · We developed CDDP-resistant A549 (A549/DDP) cells through prolonged incubation with the drug and performed RNA-seq on RNA extracts to determine … t stock the street

"WebThe IC 50 of cisplatin on A549 cells was 4.07±0.32, 2.26±0.43, and 1.52±0.41 µg/mL, for 24, 48, and 72 hours, respectively. Thus, we used 4, 2, and 1 µg/mL of cisplatin to treat A549 cells... " - Cisplatin a549 ic50

Cisplatin a549 ic50

Cathepsin L-mediated resistance of paclitaxel and cisplatin is …

WebApr 11, 2024 · Cisplatin-resistant mRNA and miRNA models were constructed by Logistic regression, classification and regression tree and C4.5 decision tree classification algorithm with previous feature selection performed via least absolute shrinkage and selection operator (LASSO). qRT-PCR and western-blotting of A549 and H358 cells, as well as … WebAug 7, 2024 · Thus, the IC50 of curcumin against A549/DDP and H1299/DDP cells was 10 μ g/mL. In addition, the maximum nonlethal concentration of curcumin against A549/DDP and H1299/DDP cells was 2.5 μ g/mL. These results showed that curcumin can significantly decrease cell viability of drug-resistant NSCLC cells. (a) (b) (c) (d) Figure 1

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WebOct 1, 2024 · Cisplatin-resistant A549 cells (A549/CisR) were derived from A549 cells by continuous cisplatin treatment. The original parental A549 cells were initially treated … WebJan 17, 2024 · A simple, sensitive and repeatable D 2 O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell metabolism. The IC 50 values obtained from A549 cells incubated with cisplatin and taxol are comparable with results of CCK-8 and ATP luminescent cell viability assays.

WebThe IC50 value of cisplatin was also assessed with CCK-8 assays. The western blotting for AKT, p-AKT, S6K and p-S6K were performed in cells with TAZ knockdown or overexpression. When the AKT/mTOR pathway was blocked in A549 and H460 with TAZ overexpression, cisplatin sensitivity was assessed by IC50 value. WebAug 1, 2024 · A549 and A549/TAX cells were seeded in 100 mm cell plate, after culturing for 24 h, CHIP assay was conducted. CHIP was performed with rabbit smad3 antibody according to the manufacturer’s protocol (Millipore, USA). Then use the TIANGEN kit to extract DNA according to the instructions. PCR was used to test the performance of the …

WebJan 17, 2024 · A simple, sensitive and repeatable D2O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell … WebCisplatin (NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP) is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy. Solutions are best fresh-prepared.

WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for …

Web0.744770. Filter: 760 entries 1 2 3 ... 31 CSV TAB XLS. The Genomics of Drug Sensitivity in Cancer Project is a collaboration between the Cancer Genome Project at the Wellcome … t stock stock price today per shareWebJul 26, 2024 · In A549 cells, cisplatin exposure led to a significantly higher expression of genes coding for proteins mediating G2/M arrest and apoptosis (mouse double minute 2 … t stocks quotesWebImmunofluorescence results elucidated that cellular ROS level in Cisplatin-induced A549 cells increases in a dose-dependent manner. Both immunofluorescence and flow cytometry results revealed that the apoptotic rate of A549 cells increases with the elevation of Cisplatin dose. phlebotomy shrewsbury hospitalWebFeb 3, 2024 · A549 and A549/CDDP cells were treated with 1/2 IC50 of cisplatin and/or PD‐0332991 for 24 h. Then cells were homogenized in RIPA buffer (50 mM Tris–HCl, pH … phlebotomy signWebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 transfection, respectively (Figure 2A). DDP with IC50 of 57.7 and 86.2 μM was obtained for A549 cells with empty vector or USP14 expression vector transduction, respectively (Figure 2B). phlebotomy simplified 2nd editionWebInhibitory concentrations 50 (IC50) and CI95 for cisplatin and compound 12 in HCT116 and A549 cells. Source publication +12 Virtual Screening and Biological Evaluation of Inhibitors Targeting... phlebotomy simplified 3rd edition pdfWebApr 13, 2024 · Cisplatin‑resistant A549 (A549/CDDP) and H460 (H460/CDDP) cells were first established and were then transfected with negative control small interfering (si)RNA (si‑NC) or TRPC1 siRNA (si‑TRPC1). ... TRPC1 silencing decreased the IC50 value of CDDP compared with the si‑NC group in A549/CDDP (11.78 vs. 21.58 µM; P<0.01) and … tsto corporation